Serological diagnosis of autoimmune bullous disease
Keywords:
serology, ELISA, autoimmune bullous diseasesAbstract
Introduction: the diagnosis of autoimmune bullous disease can be a challenging task. This motivated the implementation of serology by ELISA in the Hospital de Infecciosas Dr. Francisco J. Muñiz in 2016, method unavailable in Argentina until that moment. Objectives: to communicate the results found with the implementation of this method; to correlate these results with the clinical and epidemiological characteristics of the patients; to compute the sensibility and specificity; and to make a critical analysis of this technique. Design: cross-sectional observational analytical study. Materials and methods: serological samples of patients with bullous disease from the Department of Dermatology and other health care centers were analyzed. Time period of study: from November 2016 to August 2018. Results: 63 samples were analyzed, 40 with diagnostic of pemphigus and 23 with of epidermal-dermal junction dermatoses. The sensitivity for desmoglein 1 was 87.5%, with a specificity of 100% for the diagnosis of pemphigus. As for desmoglein 3, the sensitivity was 100% and the specificity was 92.68%. In regards to BP180 and BP230, the sensitivity was 94.11% and 17.65% and the specificity was 89.13% and 95.65%, respectively. Conclusions: this is the first national study about the use of serology by ELISA for bullous diseases. The same proved to be a practical method, easy to perform, with high sensitivity and specificity for the group of pemphigus and bullous pemphigoid. On the other hand, less conclusive results were obtained in the other entities of the epidermal-dermal junction.References
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